The laboratory study was conducted at the IRSS (Institut de Recherche en Sciences de la Santé) test facility in Burkina Faso under standard environmental conditions (27±2 °C and 75±10% relative humidity (RH)). The experimental hut trial was conducted at the field station in Vallée du Kou, an irrigated rice field area developed in 1970. The site is characterized by wooded savannah and covers 1,200 ha between 4˚24’59’’ longitude west and 11˚24’ latitude and contains seven discrete villages. Mean annual rainfall is about 1,100 mm and rice is the major crop. Few insecticides are used on this crop, but they are widely used in the surrounding villages for cotton cultivation. Thanks to irrigation, the plain provides mosquitoes with permanent, sunny, and nutrient-rich breeding sites for the development of Anopheles larvae. Mosquitoes are found year-round, but the peak density is observed in August to September during the rainy season. An. coluzzii is predominant throughout the year ²⁰ and is highly resistant to pyrethroids and dichlorodiphenyltrichloroethane (DDT) ( kdr frequency: 0.8-0.95), with a rise in ace-1 frequency also being observed ^{21– 23} . Mechanisms of metabolic resistance, such as cytochrome P450s, esterases and also non-detoxification genes have been detected ^{8, 10} . The presence of multiple resistance genes in the mosquito populations makes this area an ideal site to evaluate the effectiveness of new insecticides against mosquitoes that are resistant to conventional insecticides.

Preparation and treatment of block substrates : Two types of substrates were used to prepare IRS blocks for laboratory tests. Mud blocks were made by mixing 100g of mud and 25ml of water. The mud was from the experimental hut study site (Bama; 4°24’59” longitude west; 11°24’ latitude) to minimize variation between the mud used in laboratory and experimental hut trials. Concrete blocks were made by mixing 33g cement, 66g sand and 20mL water. Blocks were shaped in Petri dishes (9 cm diameter and 1 cm thick). Mud blocks and concrete blocks were left to dry for a minimum of 1 week and for 1 month, respectively, at 27 °C ± 2 °C and 75% ± 10% relative humidity before insecticide was applied. The pH of the concrete blocks was tested on the day they were to be sprayed by scraping 5g of concrete from a block, adding 15ml distilled water, mixing thoroughly, and measuring with a pH meter (HANNA Instruments, model Hi 9813-5): blocks with a pH between 6-10 (mud and concrete) were judged suitable for use. The blocks were sprayed with the different treatments ( Table 1) using a homogeneous solution of each dose. The VECTRON™ T500 product as batch no 18I-3671 was provided by Mitsui Chemicals Agro, Inc. (MCAG). Spraying was done using a calibrated Potter Precision Laboratory Spray Tower (Burkard Manufacturing Co Ltd, Rickmansworth, UK) which is internationally recognized as the most precise method of chemical spraying in the laboratory as described in WHO testing guidelines ²⁴ . All treated blocks were stored at 30 °C ± 2 °C and 75% ± 10% RH in between bioassays. In total, five blocks of each substrate type were prepared and sprayed for each dose.

Residual efficacy of broflanilide WP (VECTRON™ T500) in laboratory cone bioassays : After spraying, WHO cone bioassays were performed according to WHO guidelines ²⁴ to evaluate the residual activity of insecticide on the substrates. Bioassays were performed at 1 week and then monthly up to 14 months post spraying, by attaching the cones to the treated and control blocks. For each insecticide dose and substrate type used, 100 unfed female mosquitoes aged 2 to 5 days were exposed in WHO polyvinyl chloride cones (obtained from the Vector Control Research Unit (VCRU) WHO Collaborating Centre, Universiti Sains Malaysia, Penang, Malaysia) for 30 minutes contact time with 10 mosquitoes per cone per block. Two cone bioassays were performed per block, with five blocks of each treatment. An. gambiae Kisumu susceptible strain and An. coluzzii VK laboratory resistant strain, reared at the IRSS insectary under standard controlled conditions (27±2°C and 75±10% relative humidity), were used. After removal from cones, mosquitoes were transferred into holding cups, provided access to 10% sucrose soaked cotton wool, and held under the same conditions described earlier. Mortality was recorded at 24 hours, 48 hours and 72 hours post exposure in cones.

Design of huts : The experimental huts used were of the West African design ²⁵ . An experimental hut is a simulated house in which all entering, exiting (exophily), dead and blood fed mosquitoes can be recorded. It is made of local material and is characterized by the presence of a gutter or moat around the hut to protect against ants which would eat dead mosquitoes. It is also characterized by the presence of veranda traps to catch mosquitoes which may exit during the night due to either behavioural or insecticidal effects. Mosquitoes can enter through four window slits constructed from pieces of metal, fixed to create a funnel of 1 cm wide gap to inhibit mosquitoes from exiting. The ceiling of the huts was made of plastic. For interior wall surfaces, two types of material were used: concrete and mud.

Treatments : VECTRON™ T500 was evaluated at two application rates (100 mg a.i./m ² and 150 mg a.i./m ² rather than 200mg/m ²) tested in laboratory) on concrete and mud walls in the experimental huts. The reference product was Actellic® 300CS (Syngenta), which contains the organophosphate insecticide pirimiphos-methyl as the active ingredient, It was used at the recommended dose of 1000 mg a.i./m ². A negative control, sprayed only with distilled water, was also included. Table 2 below summarises the different treatment arms and substrates that were tested.

Insecticide application : The IRS treatments were applied at the specified dosages ( Table 2) to the internal walls of experimental huts and the hut ceiling using a MICRON CS-10 10L compression sprayer, fitted with a red 4.2 bar CFV and a T-Jet 8002E flat fan nozzle. The target volume ejected was 560 mL/min. All sprayers were calibrated with water prior to treatment of huts. All sprayers were equipped with pressure gauges, and initial pressure settings were conducted at 60 psi for consistency. The huts were prepared before spraying by marking swaths on the walls and ceiling, each swath being 75 cm in width and with a 5 cm overlap with the next swath. The safety precautions, mixing, handling, spray techniques and spray tank washing were all done according to standard procedures as outlined in the WHO manual for IRS. Prior to spraying, the spray operator practiced several times on blank walls using a tank filled with water to ensure that a constant flow rate was obtained before treatment started. A digital metronome (freeware from Metronome Beats v. 2.3.3, Stonekick 2013) synchronized with a digital stopwatch was used to enhance the consistency of applications (6 seconds per spray swath). The use of the digital metronome provided an audible guide to spray operators. An orientation pole was attached to the handle of the sprayer during spraying to maintain the correct distance of the sprayer nozzle from the walls. It was attached to the handle of the sprayer during spraying. Five labelled filter papers (Whatman™ No. 1 10cm × 10cm) were fixed onto the four walls and ceiling of the huts. The filter papers were removed after spraying, dried, grouped by hut and treatment, and carefully packed in aluminium foil for subsequent High performance Liquid Chromatography (HPLC) analysis at the Liverpool School for Tropical Medicine (LSTM), to provide a measure of the quality of the treatment applications. Insecticides were mixed homogeneously in the spray tank. Spraying was done alternately from roof to floor and then from floor to roof to treat each hut. After spraying the wall, the tarpaulin ceiling previously arranged on a plastic support was also sprayed. The sprayer tank was shaken frequently to ensure proper mixing. After spraying of each treatment, the solution remaining in the pump was removed and the volume measured. This measurement made it possible to determine the actual quantity of treatment solution applied per hut.

Trial procedure : Evaluation of free flying mosquitoes started five days after applying the treatments inside the 6 huts. Cows were used as bait for mosquito attraction in place of human volunteers as the local An. coluzzii population is relatively zoophilic (according to unpublish previous trials data) and also the toxicity, and potential risk of the VECTRON™ T500 product had not been fully assessed at the time of study initiation. In total, 14 cows, male and female, aged between 2 and 3 years, were purchased locally. A veterinarian was recruited to follow their health. They were used in this study according to his requirements to ensure their good health. Cows were divided in two groups (7 per group): one group (6 cows and 1 on back up) was used during one week and the second during the following week. The cows of each group were randomized on the first day of use and placed inside the huts in containment crates made of wood. Cows were rotated between huts each night according to a Latin square design to control for any variation in the attractiveness of individual cows to the mosquitoes. Thus, every cow spent one night in each hut during the round of 6 nights. They were placed inside huts at dusk (7:00 pm) and remained inside until dawn.

Each morning, volunteers, recruited from the village around the huts station and trained in mosquito collection, entered the huts to collect the mosquitoes that had entered overnight. Dead and live mosquitoes were collected from the floor, the walls and the ceiling of the hut and from the veranda trap, and placed into collection tubes. Mosquitoes were put in different bags for each collection compartment and transferred to the laboratory. Species identifications were made using the appropriate taxonomic keys. Mosquitoes were scored by location as dead or alive and as fed or unfed. Live mosquitoes were placed in cups covered with clean netting and provided with a 10% glucose solution for assessment of delayed mortality up to 72 hours after collection. Trial was run on 12 rounds of 6 days each.

The main outcomes measured were:

Evaluation of insecticide residual activity using cone tests : Residual activity of the different treatments was assessed at 1 week and then monthly after spraying up to 9 months for the VECTRON™ T500 100 mg a.i./m² and Actellic® 300CS treatments and up to 12 months for the VECTRON™ T500 150 mg a.i./m² treatment. Females of the susceptible strain An. gambiae Kisumu strain and the resistant strain An. coluzzii VK strain were tested using WHO standard cone bioassays on the treated walls and ceiling ²⁴ . Two cones were attached with masking tape on each of the hut inner walls (the four walls and the ceiling) to obtain ten (10) cones per hut. Ten (10) females were exposed in each cone by plugging the cone with cotton wool after the introduction of mosquitoes. After 30 minutes of contact, mosquitoes were removed and placed in 150-ml plastic cups with access to the glucose solution (10%) provided via cotton wool. Mosquitoes were quickly transferred to the holding room in Bobo-Dioulasso (30 min drive) and maintained at a temperature of 27°C ± 2°C and 75% ± 10% RH. Knockdown was recorded 60 minutes after exposure, and mortality was recorded 24, 48 and 72 hours after exposure in cones.

The aim of this chemical analysis was to assess the quality of the spraying by comparing the doses of insecticides on the papers with the target doses. The difference of the two doses in percentage should be in the range of ±50% of the target dose according to WHO recommendation ²⁶ .

The five labelled filter papers (10 cm × 10 cm) fixed to the walls and ceiling of each hut before spraying were removed after spraying, packed in aluminium foil separately, and put in labelled bags. The packed samples were stored in a refrigerator at +4°C temperature and were then shipped to LSTM/LITE in Liverpool for HPLC analysis.

Broflanilide content was determined by reversed-phase high-performance liquid chromatography (HPLC) using UV detection at 226 nm and dicyclohexyl phthalate (DCP) standard as an internal standard. Briefly, a hole punch (0.635cm radius) was used to cut 12 circles from each filter paper. The pieces of each filter were placed into a glass tube and 5 ml of extraction solution was added, consisting of 100 µg/ml of DCP in methanol. The glass tubes were capped with tin foil and a screw cap and placed into a water bath sonicator. Samples were sonicated for 60 minutes at room temperature. Once sonication was completed, a syringe and PTFE filter (0.2 µm) was used to transfer 1 ml of each solution to a 1.5 ml Eppendorf tube. Using a 200µl micropipette, a 100µl aliquot of each sample was pippeted into a labelled HPLC vial. The HPLC was equipped with a detector suitable for operation at 226 nm, a constant temperature column compartment and an injector capable of delivering 20 µl injection volume. A Thermo Scientific Hypersil Gold column (Thermo Fisher Scientific, U.K.; Particle size: 5µm) was attached to the HLPC equipment. The mobile phase was acetonitrile and water mixed in a 7:3 ratio (v/v) with a flow rate of 1 ml/min and detection at 226 nm. The column temperature was between 23°C and 25°C.

A sub-sample of mosquitoes (610 individual mosquitoes) collected in treated huts were submitted for polymerase chain reaction (PCR) analysis to determine species and the presence of kdr resistance by genotyping. The cycling conditions were 10' [30",30",60"] 35c @ 54°C for Sine and 3' [30", 30", 10"] 35c @ 55°C for Kdr_w. The reagents and kits (details in supplementary file 11, Extended data ²⁷ ) used are Pool Master Mix, Primers, sterile water, Trizma base, boric acid, EDTA, Agarose Multi, Purpose Agarose, Hexadecyltrimethylammonium bromide, sodium chloride, Trizma hydrochloride solution Ph 8.0, 1M and Ethylenediaminetetraacetic acid, 0,5 M aq. Soln, pH 8.0 Liquid. The primers were: S200X 6.1F: TCG-CCT-TAG-ACC-TTG-CGT-TA, S200X 6.1R: CGC-TTC-AAG-AAT-TCG-AGA-TAC, Kdr_w D1: ATA-GAT-TCC-CCG-ACC-ATG, Kdr_w D2: AGA-CAA-GGA-TGA-TGA-ACC, Kdr_w D3: AAT-TTG-CAT-TAC-TTA-CGA-CA and Kdr_w D4: CTG-TAG-TGA-TAG-GAA-ATT-TA. The main equipments were composed of by thermocyclers (Eppendorf, Biorad, Applied Biosystems), transluminator, migration cuve (Fisherbrand, Apelex), vortex, centrifuges, Eppendorf pipettes, Electrophoresis Power Supply (E 815 CNSort and E 844 CNSort) (details in supplementary file 11, Extended data ²⁷ ). The species identification to identify An. gambiae complex species used the standard protocol ²⁸ and the presence/absence of kdr mutation L1014F (kdr-w) was determined using the protocol described by Martinez-Torres et al. ²⁹ . Mosquitoes were identified as An. arabiensis, An. gambiae sensu stricto or An. coluzzii. For the resistance assays, mosquitoes were classified as SS, RS or RR i.e., homozygous susceptible, heterozygous, or homozygous resistant for the kdr mutation L1014F.

Phenotypic resistance was evaluated using 2 to 5 -day old adult female mosquitoes according to the standard WHO susceptibility test method ³⁰ . The insecticide and PBO treated papers (impregnated papers) were obtained from WHO laboratory in Malaysia.

WHO cone bioassay and experimental hut trial data were entered using EpiData v 3.1 software (RRID:SCR_008485). Mortality was calculated from the total number of mosquitoes tested per period. If the mortality of the negative control in WHO cone bioassay was between 5% and 20%; mortality of treated mosquitoes was corrected using Abbott’s formula.

Abbott’s formula: Corrected mortality = (% treated mortality − % negative control mortality) 100 − % negative control mortality × 100

If negative control mortality was above 20% at 24 hours after exposure, the test data for that day was discarded, and the cone bioassays repeated. In the experimental hut trial, the free flying mosquito data such as the number of mosquitoes that entered, exited, dead inside the hut. or during 72 hours observation time were recorded. The number that succeeded in blood feeding on the cows were calculated for each treatment by compiling the data collected over 12 weeks. The main analyses were performed using R statistical software (RRID:SCR_001905) version 4.1.0 with a significance level of 0.05 for rejecting the null hypothesis following a predefined analysis plan. Mixed effect logistic regression model analysis was conducted using the lme4 package, to compare proportional data by taking mosquitoes exited, blood fed and dead (total mortality) as dependent variables and treatment as categorical covariates (fixed effect), sleepers (cows) and months of the trial (random effect). For overall comparison, the negative control (untreated hut) was kept as a reference category. The primary criteria in the evaluation were blood feeding inhibition and 72 hours mortality. All graphs were produced using Excel 2016.

Institutional ethical approval for the study was obtained on October 2016 from the Institutional (Institut de Recherche en Sciences de la Santé) Ethics Committee for Health Sciences Research (N/Réf. 023- 2016/CEIRES). The cows used in experimental huts to attract mosquitoes were maintained according to the institution's recommendations. Care was taken that the cows were not traumatized. A veterinarian was recruited to monitor their hygiene and health. All sick cows were replaced and treated appropriately. During the day, the cows were allowed to graze freely in an open field. The study was performed according to relevant international animal use guidelines ³¹ . This manuscript is reported in line with the ARRIVE (Animal Research: Reporting of In Vivo Experiments) guidelines ³² .

The residual efficacy of VECTRON™ T500 was investigated at application rates of 50, 100 and 200 mg a.i./m² on concrete and mud blocks under laboratory conditions. On concrete blocks, the 50 mg a.i./m ² and 100 mg a.i./m ² application rates of VECTRON™ T500 resulted in 100% mortality for up to 12 months before falling below 80% during the last two months, while the 200 mg a.i./m ² dose of VECTRON ^TM T500 gave complete mortality of susceptible An. gambiae s.s. Kisumu strain for up to 14 months ( Figure 1a) ²⁷ . VECTRON™ T500 on mud blocks showed a longer residual efficacy than on concrete blocks. Indeed, as shown in Figure 1b, all applications to mud blocks induced 100% mortality for up to 14 months after spraying with the An. gambiae s.s. Kisumu strain ( Figure 1b).

The residual efficacy of VECTRON™ T500 against the pyrethroid resistant VK strain of Anopheles coluzzii is shown in Figure 2. The results of mortality following exposure to treated concrete blocks showed dose dependent response. Indeed, for the 50 mg a.i./m ² application rate, the mortality was around 80% at 5 months after block treatment before decreasing below 60% during the last six months. However, at the highest doses, the mortality rate was still high at 10 months after spraying, reaching 89.81% and 100% for the 100 mg a.i./m ² and 200 mg a.i./m ² doses, respectively, before decreasing below 80% during the last three months ( Figure 2a). As with the susceptible An. gambiae s.s. Kisumu strain, VECTRON™ T500 showed better residual efficacy on mud blocks compared with concrete blocks against the resistant VK strain of Anopheles coluzzii. Indeed, 100% mortality was recorded up to 14 months for the 200 mg a.i./m ² application rate. The lowest application rate (50 mg a.i./m ²) demonstrated good residual efficacy up to 8 months after spraying with mortality up to 98.16% during that period ( Figure 2b). The 100 mg a.i./m ² application rate induced mortality up to 92% 14 months after spraying, although there was some variation in mortality between months 10 and 13 ( Figure 2b).

Efficacy of VECTRON ^TM T500 against free flying mosquitoes

Residual efficacy of VECTRON ^TM T500 against wild free flying pyrethroid resistant malaria vectors was investigated in an experimental hut trial at the IRSS field station in Vallée du Kou (Bama, Burkina Faso). Experimental huts simulate the conditions in domestic dwellings and are, therefore, used to assess the efficacy of indoor vector control interventions in terms of mosquito entry rates, induce early exit of vector mosquitoes, prevention of mosquito feeding and induced mosquito mortality. Cows were used in place of human volunteers for mosquito attraction in this study as mosquitoes are zoophilic and also the toxicity, and potential risk of the VECTRON ^TM T500 product had not been fully assessed at the time of study initiation. In addition, previous studies have shown that the local vector mosquitoes are highly attracted to blood-feed on cows. Results of the different outcome measures are presented in Figure 3 and Table 3. In total 19,552 An. gambiae s.l. mosquitoes were collected between August and October 2018. Mortality of free flying mosquitoes was recorded up to 72 hours after collection from huts, due to the delayed mortality effect of brofanilide, the active ingredient of VECTRON ^TM T500,Mortality rates of free flying An. gambiae s.l. indicate that the 150 mg a.i./m ² dose of broflanilide was the most effective in killing mosquitoes. Indeed, this dose induced the highest overall mosquito mortality rates on concrete (70.04%) and on mud (73.22%) during the four months of mosquito collection post spraying. Statistically, the 150 mg a.i./m ² dose performed significantly better (P<0.001) on mud than on concrete. During this period, mortality with the 150 mg a.i./m ² dose of VECTRON™ T500 ranged from 59.74% to 79.06% on concrete walls and from 55.33% to 79.33% on mud walls ( Figure 3). Mortality of mosquitoes collected in the huts treated with 100 mg a.i./m² VECTRON™ T500 ranged from 54.57% to 72.87% on concrete walls and from 40.31% to 63.54% on mud walls with 60.40% and 55.51% as global mortality, respectively ( Figure 3 and Table 3). In contrast, 100 mg a.i./m² dose of VECTRON™ T500 performed significantly better (P<0.0001) on concrete than mud. On both substrates, there was a significant difference between150 mg a.i./m² dose and 100 mg a.i./m² in terms of mortality (P<0.0001). The positive reference product, Actellic® 300CS, induced 100% mortality during the four months of evaluation. Deterrence, blood-feeding inhibition and exophily obtained with VECTRON™ T500 treated huts compared to negative control were very low ( Table 3). The lowest of these parameters can be explained by the low action of insecticide on the mosquitoes. The Actellic® 300CS treatment showed 55% deterrence. As expected of IRS treatments, blood-feeding rates of mosquitoes were very high in all huts (>90%). There was a significant difference in blood-feeding rates between the two application rates of VECTRON™ T500 (100 mg a.i./m² and 150 mg a.i./m²) for both concrete and mud substrates (P<0.05, Table 3). The natural exophily rate in the control hut was high. Due to this natural exophily, it was not possible to determine the insecticide-induced exophily.

Residual efficacy of insecticide applied in experimental huts using cone tests

Cone bioassays were performed monthly in experimental huts up to 9 months for the 100 mg a.i./m ² application rate of VECTRON™ T500 and for the Actellic® 300CS treatment. The 150 mg a.i. /m ² application rate of VECTRON™ T500 was evaluated up to 12 months after spraying, to assess the residual efficacy on the different hut wall substrates (mud and concrete). Unfed adult females (3–5 days old) of the susceptible An. gambiae Kisumu and resistant An. coluzzii (reared from larvae collected at the experimental field site) were used. The residual efficacy in cone bioassays with the susceptible Kisumu strain is presented in Figure 4. The 100 mg/m ² and 150 mg/m ² doses of VECTRON™ T500 induced 100% mortality on both concrete and mud walls up to 9 months after spraying. The three extended monthly test performed with 150 mg/m² showed better efficacy up to 12 months on mud walls (100%) than concrete walls (<80%). The Actellic® 300CS reference product applied to concrete showed variable mortality from 4 months post-treatment onwards, and mortality at 6 and 7 months was below 80%. Residual efficacy against the pyrethroid resistant VK strain An. coluzzii is shown in Figure 5. The 100 mg a.i./m² and 150 mg a.i./m ² doses of VECTRON™ T500 performed better on mud walls (mortality over 80%) 9 months after spraying than on concrete walls (mortality below 80%) at the same time. The Actellic® 300CS showed variable residual efficacy from 4 months post-treatment onwards, but mortality was below 80% at 8 and 9 months.

The results of the HPLC analysis of filter papers treated during the application of treatments to experimental hut walls are shown in Table 4. The percentage difference between the target dose and the actual dose sprayed onto filter papers was within the range of ±50% of the target doses for all treatments confirming that the spraying met WHO statement for spray quality ²⁶ .

To determine the prevalence of phenotypic resistance, larvae were collected from breeding sites near to the experimental hut station and reared in the insectary to adults 2–5 days old. WHO susceptibility bioassays were performed using insecticide impregnated papers obtained from the Vector Control Research Unit (VCRU) WHO Collaborating Centre, University Sains Malaysia, Penang, Malaysia in Malaysia. The insecticide susceptible Kisumu strain of An. gambiae s.s. was also tested for data quality control purposes. The results summarized below ( Table 5) showed full susceptibility (100% mortality) of the Kisumu strain and a very high level of resistance to pyrethroids (<2% mortality) in the field population of An. gambiae s.l. The increase in mortality (39%) with deltamethrin following exposure to the cytochrome P450 synergist piperonyl butoxide (PBO), indicates the role of a P450 metabolic mechanism of pyrethroid resistance in this population. Resistance was also observed in the field population to the carbamate bendiocarb (84%), but it was fully susceptible to the organophosphorus insecticide pirimiphos-methyl.

Mosquitoes sampled from treated huts (610 individual mosquitoes) were used in PCR tests to determine species and to detect kdr resistance mutations. Of these mosquitoes, 8 mosquitoes did not amplify. A high proportion of the mosquitoes collected from huts were An. coluzzii (98%), and these had a high frequency of the kdr (L1014F) mutation (0.65). The proportion of heterozygote, homozygote resistance and homozygote susceptible was 42.6%, 44.5% and 12.8% respectively ( Table 6). The kdr mutations confer cross-resistance between pyrethroids and DDT in these mosquitoes.

Zenodo: koamabayili/VECTRON: Laboratory and experimental hut trial evaluation of VECTRON™ T500 for indoor residual spraying (IRS) against insecticide resistant malaria vectors in Burkina Faso. https://doi.org/10.5281/zenodo.6469836 ²⁷ .

This project contains the following underlying data:

Zenodo: koamabayili/VECTRON: Laboratory and experimental hut trial evaluation of VECTRON™ T500 for indoor residual spraying (IRS) against insecticide resistant malaria vectors in Burkina Faso. https://doi.org/10.5281/zenodo.6469836 ²⁷ .

This project contains the following extended data:

Zenodo: ARRIVE checklist for ‘Laboratory and experimental hut trial evaluation of VECTRON™ T500 for indoor residual spraying (IRS) against insecticide resistant malaria vectors in Burkina Faso’.

https://doi.org/10.5281/zenodo.6469817 ³² .

Data are available under the terms of the Creative Commons Zero "No rights reserved" data waiver (CC0 1.0 Public domain dedication).